Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction

作者：Madwar Carolin; Kwan William Chu; Deng Lingquan; Ramstrom Olof; Schmidt Rolf; Zou Shan; Cuccia Louis A^*

来源：Langmuir, 2010, 26(22): 16677-16680.

DOI：10.1021/la1036579

The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomoleculcs and polymers to surfaces. Herein, it single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded in average unbinding force of 70-80 pN for loading rates between 8000 and 40 000 pN/s for mannose surfaces on aminated glass. and an unbinding force of 57 +/- 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k(off), for this interaction to be 0.16 s(-1).

• 出版日期2010-11-16