Modification of Decellularized Goat-Lung Scaffold with Chitosan/Nanohydroxyapatite Composite for Bone Tissue Engineering Applications

作者：Gupta Sweta K; Dinda Amit K; Potdar Pravin D; Mishra Narayan C^*

来源：Biomed Research International, 2013, 2013: 651945.

DOI：10.1155/2013/651945

Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue, and the scaffold was modified with chitosan/nanohydroxyapatite composite for the purpose of bone tissue engineering applications. MTT assay with osteoblasts, seeded over the chitosan/nanohydroxyapatite-modified decellularized scaffold, demonstrated significantly higher cell growth as compared to the decellularized scaffold without modification. SEM analysis of cell-seeded scaffold, after incubation for 7 days, represented a good cell adhesion, and the cells spread over the chitosan/nanohydroxyapatite-modified decellularized scaffold. Expression of bone-tissue-specific osteocalcin gene in the osteoblast cells grown over the chitosan/nanohydroxyapatite-modified decellularized scaffold clearly signifies that the cells maintained their osteoblastic phenotypewith the chitosan/nanohydroxyapatite modified decellularized scaffold. Therefore, it can be concluded that the decellularized goat-lung scaffold-modified with chitosan/nanohydroxyapatite composite, may provide enhanced osteogenic potential when used as a scaffold for bone tissue engineering.

• 出版日期2013
• 单位河北医科大学