Differential expression of protein kinase C alpha and delta in testes of mouse at various stages of development

作者：Feng C; Zhang J; Gasana V; Fu W; Liu Y; Zong ZH; Yu BZ^*

来源：Cell Biochemistry and Function, 2005, 23(6): 415-420.

DOI：10.1027/cbf.1167

• protein kinase C
• testis
• development

Protein kinase C (PKC) describes a family of serine/threonine protein kinases, and multiple isoforms are expressed in various mammalian tissues. In the present study, we examined the expression of PKC-alpha and PKC-delta at protein and mRNA level in mouse testis by Western blotting and RT-PCR. We also examined the expression of both PKC isoenzymes in the developing mouse testis. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC-alpha were uniformly distributed; but PKC-delta expression occurred in the testes of 3-week-oldmice, perhaps even at a relatively late stage in spermatid development. The results suggest that each isoenzyme may have different functional roles in processing and modulating physiological cellular responses of spermatogenesis.

• 出版日期2005-12
• 单位中国医科大学