Approach to develop a standardized TLC-DPPH center dot test for assessing free radical scavenging properties of selected phenolic compounds

作者：Ciesla Lukasz^*; Kryszen Jakub; Stochmal Anna; Oleszek Wieslaw; Waksmundzka Hajnos Monika

来源：Journal of Pharmaceutical and Biomedical Analysis, 2012, 70: 126-135.

DOI：10.1016/j.jpba.2012.06.007

• TLC-DPPH center dot
• Thin-layer chromatography
• Free radical scavengers
• 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH center dot)
• Phenolic compounds

TLC-DPPH center dot test belongs to a group of frequently performed assays aimed at detection of compounds with desired activity (effect directed analysis). Despite its popularity a standard procedure has not been elaborated so far causing difficulties in comparing results obtained in different laboratories. Thus the aim of the presented research was an approach to develop a standardized procedure for assessing free radical scavenging properties of plant polyphenols. It was observed that specifically positive adsorbent (silica gel) strengthened the observed result of radical-antioxidant reaction, while polar bonded stationary phase CN-silica weakened it. Based on the observed results it was concluded that the TLC-DPPH center dot assay should be preferably performed on the surface of non-specific adsorbents (e.g.: RP-18) with the use of n-hexane for DPPH center dot dissolution. It is also proposed to document the results every 5 min after staining, as they change in time.

• 出版日期2012-11