Activation of Olfactory Receptors on Mouse Pulmonary Macrophages Promotes Monocyte Chemotactic Protein-1 Production

作者：Li Jing Jing; Tay Hock L; Plank Maximilian; Essilfie Ama Tawiah; Han**ro Philip M; Foster Paul S^*; Yang Ming

来源：PLos One, 2013, 8(11): e80148.

DOI：10.1371/journal.pone.0080148

Background: Emerging evidence suggests that non-olfactory tissues and cells can express olfactory receptors (ORs), however, the exact function of ectopic OR expression remains unknown. We have previously shown in mouse models that a unique cooperation between interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) drives the activation of pulmonary macrophages and leads to the induction of pathogenic responses in the respiratory tract. Further, through gene array studies, we have shown that activation of macrophages by these molecules results in the selective expression of a number of ORs. In this study, we validated the expression of these ORs in mouse airway and pulmonary macrophages in response to IFN-gamma and LPS (gamma/LPS) stimulation, and further explored the effect of odorant stimulation on macrophage function. %26lt;br%26gt;Methodology/Principal Findings: OR expression in airway and pulmonary macrophages in response to IFN-gamma, LPS or gamma/LPS treatments was assessed by microarray and validated by q-PCR. OR expression (e.g. OR622) on macrophages was confirmed by visualization in immunofluoresence assays. Functional responses to odorants were assessed by quantifying inflammatory cytokine and chemokine expression using q-PCR and cell migration was assessed by a modified Boyden chamber migration assay. Our results demonstrate that eight ORs are expressed at basal levels in both airway and pulmonary macrophages, and that gamma/LPS stimulation cooperatively increased this expression. Pulmonary macrophages exposed to the combined treatment of gamma/LPS+ octanal (an odorant) exhibited a 3-fold increase in MCP-1 protein production, compared to cells treated with gamma/LPS alone. Supernatants from gamma/LPS+ octanal exposed macrophages also increased macrophage migration in vitro. %26lt;br%26gt;Conclusions/Significance: Eight different ORs are expressed at basal levels in pulmonary macrophages and expression is upregulated by the synergistic action of gamma/LPS. Octanal stimulation further increased MCP-1 production and the motility of macrophages. Our results suggest that ORs may mediate macrophage function by regulating MCP-1 production and cell migration.

• 出版日期2013-11-21