Analysis of Binding Interaction Between Prulifloxacin and Pepsin in the Existence of Eu(III): A Spectroscopic Analysis

作者：Gao, Xiaoyan; Zhang, Xiaojie; Tang, Yingcai; Zhang, Xiaoping; Zhao, Wujie; Zi, Yanqin^*

来源：Current Analytical Chemistry, 2011, 7(3): 194-200.

DOI：10.2174/1573411011107030194

• PUFX
• Pepsin
• Fluorescence quenching
• Binding constant
• Eu(III)

Interaction between prulifloxacin (PUFX) and pepsin was studied under a simulated physiological condition using fluorescence spectrophotometric techniques when the Eu(III) exists. The fluorescence emission intensity of pepsin was strongly quenched by the addition of prulifloxacin. Spectrophotometric observations are rationalized in terms of a static quenching process. The binding constants and the number of binding sites have been evaluated by the data obtained from the fluorescence quenching experiments. According to the van't Hoff equation, the standard enthalpy change (Delta H-0) and standard entropy change (Delta S-0) for the reaction were calculated and it indicated that the hydrophobic interactions play a main role in the binding of PUFX and pepsin. In addition, the binding distance R between pepsin (donor) and PUFX (acceptor) was obtained on the basis of the Forster's resonance energy transfer theory. The results obtained herein show that the interaction between the pepsin and PUFX was not influenced by Eu(III).

• 出版日期2011-7
• 单位淮北师范大学; 宿州学院; 苏州大学