Morphological alterations of Vero cell exposed to coplanar PCB 126 and noncoplanar PCB 153

作者：Shen Kaili; Shen Chaofeng; Chen Lei; Chen Xincai^*; Chen Yingxu

来源：Environmental Toxicology, 2012, 27(1): 26-31.

DOI：10.1002/tox.20608

• PCBs
• Vero cell
• cytotoxicity
• apoptosis

Polychlorinated biphenyls (PCBs) are widespread, persistent environmental contaminants that display a complex spectrum of toxicological properties. Exposure to PCBs has been associated with morphological anomalies in cell cultures. However, most mechanistic studies of PCBs' toxic activity have been focused on coplanar congeners. It is of importance to determine whether PCB treatment would influence cell configuration and whether these changes would depend on the structural characteristics of PCBs. In this study, we investigated cell morphological alteration in Vero cell cultures after exposure to coplanar PCB 126 and noncoplanar PCB 153. The survival of Vero cells was measured through the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test. Cytotoxicity results suggested that PCB congeners had a toxic, antiproliferative effect on Vero cells. Morphological studies described structural modifications and provided evidence that apoptosis might be the main cell death pathway in PCB 153-treated cells. The comparison between PCB 126 and PCB 153 indicated that the cell death mechanisms involved in coplanar or noncoplanar PCB congener exposure were different in Vero cells.

• 出版日期2012-1
• 单位浙江大学