Detection of plaque structure and composition using OCT combined with two-photon luminescence (TPL) imaging

作者：Wang Tianyi^*; McElroy Austin; Halaney David; Vela Deborah; Fung Edmund; Hossain Shafat; Phipps Jennifer; Wang Bingqing; Yin Biwei; Feldman Marc D; Milner Thomas E

来源：Lasers in Surgery and Medicine, 2015, 47(6): 485-494.

DOI：10.1002/lsm.22366

• atherosclerotic plaque
• lipid
• oxidized-LDL
• calcium
• collagen
• elastin fiber
• hybrid imaging
• optical coherence tomography
• two-photon luminescence imaging
• photonic crystal fiber

Background and ObjectivesAtherosclerosis and plaque rupture leads to myocardial infarction and stroke. A novel hybrid optical coherence tomography (OCT) and two-photon luminescence (TPL) fiber-based imaging system was developed to characterize tissue constituents in the context of plaque morphology.
Study Design/Materials and MethodsEx vivo coronary arteries (34 regions of interest) from three human hearts with atherosclerotic plaques were examined by OCT-TPL imaging. Histological sections (4m in thickness) were stained with Oil Red O for lipid, Von Kossa for calcium, and Verhoeff-Masson Tri-Elastic for collagen/elastin fibers and compared with imaging results.
ResultsBiochemical components in plaques including lipid, oxidized-LDL, and calcium, as well as a non-tissue component (metal) are distinguished by multi-channel TPL images with statistical significance (P<0.001). TPL imaging provides complementary optical contrast to OCT (two-photon absorption/emission vs scattering). Merged OCT-TPL images demonstrate the distribution of lipid deposits in registration with detailed plaque surface profile.
ConclusionsResults suggest that multi-channel TPL imaging can effectively identify lipid sub-types and different plaque components. Furthermore, fiber-based hybrid OCT-TPL imaging simultaneously detects plaque structure and composition, improving the efficacy of vulnerable plaque detection and characterization. Lasers Surg. Med. 47:485-494, 2015.

• 出版日期2015-8