DEHP exposure impairs mouse oocyte cyst breakdown and primordial follicle assembly through estrogen receptor-dependent and independent mechanisms

作者：Mu Xinyi; Liao Xinggui; Chen Xuemei; Li Yanli; Wang Meirong; Shen Cha; Zhang Xue; Wang Yingxiong; Liu Xueqing; He Junlin^*

来源：Journal of Hazardous Materials, 2015, 298: 232-240.

DOI：10.1016/j.jhazmat.2015.05.052

• DEHP
• Primordial follicles assembly
• Estrogen receptor
• Notch signaling
• Pregranulosa precursor cell

Estrogen plays an essential role in the development of mammalian oocytes, and recent studies suggest that it also regulates primordial follicle assembly in the neonatal ovaries. During the last decade, potential exposure of humans and animals to estrogen-like endocrine disrupting chemicals has become a growing concern. In the present study, we focused on the effect of diethylhexyl phthalate (DEHP), a widespread plasticizer with estrogen-like activity, on germ-cell cyst breakdown and primordial follicle assembly in the early ovarian development of mouse. Neonatal mice injected with DEHP displayed impaired cyst breakdown. Using ovary organ cultures, we revealed that impairment was mediated through estrogen receptors (ERs), as ICl 182,780, an efficient antagonist of ER, reversed this DEHP-mediated effect. DEHP exposure reduced the expression of ER beta, progesterone receptor (PR), and Notch2 signaling components. Finally, DEHP reduced proliferation of pregranulosa precursor cells during the process of primordial folliculogenesis. Together, our results indicate that DEHP influences oocyte cyst breakdown and primordial follicle formation through several mechanisms. Therefore, exposure to estrogen-like chemicals during fetal or neonatal development may adversely influence early ovarian development.

• 出版日期2015-11-15
• 单位重庆医科大学