Microsatellite markers for Hebeloma species developed from expressed sequence tags in the ectomycorrhizal fungus Hebeloma cylindrosporum

作者：Jany JL^*; Bousquet J; Khasa DP

来源：Molecular Ecology Notes, 2003, 3(4): 659-661.

DOI：10.1046/j.1471.8286.2003.00537.x

• ectomycorrhizal fungus
• expressed sequence tags
• Hebeloma cylindrosporum
• microsatellite

The increasing number of expressed sequence tag (EST) projects dedicated to ectomycorrhizal fungi is translating into the release of large sets of ESTs. The aim of this study was to develop and test simple sequence repeat (SSR) markers from EST databases of the model ectomycorrhizal fungus Hebeloma cylindrosporum. Six SSR markers were found to be both unambiguously scorable and polymorphic among 12 H. cylindrosporum isolates. Two SSR markers were transferable to other Hebeloma species and one marker was interestingly found to be polymorphic among seven H. crustuliniforme isolates.

• 出版日期2003-12