Atomic structure of the Y complex of the nuclear pore

作者：Kelley Kotaro; Knockenhauer Kevin E; Kabachinski Greg; Schwartz Thomas U^*

来源：Nature Structural & Molecular Biology, 2015, 22(5): 425-U108.

DOI：10.1038/nsmb.2998

The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on similar to 15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-angstrom crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire similar to 0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.

• 出版日期2015-5