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摘要
In this work a very simple and rapid TLC densitometric method used to quantitative analysis of ursodeoxycholic acid in commercial drugs was presented. Ursodeoxycholic acid (UDCA) was effective extracted from pharmaceutical formulations in form of tablets and capsules by means of methanol. Chromatography was performed on glass plates precoated with silica gel 60F254 (E. Merck, Art. 1.05715) with the use of mixture: n-hexane-ethyl acetate-acetic acid in volume composition 22:22:5 as a mobile phase. Under these optimum conditions the RF value for ursodeoxycholic acid is equal to 0.48. After visualization of spots with the use of 10% H2SO4 and next heating them in temp. 120 degrees C, the chromatograms were quantitative scanned by densitometer at maximum wavelength =360nm. On the basis of obtained results it was stated, that elaborated method can be used in routine quantity control of ursodeoxycholic acid in selected pharmaceutical formulations.
- 出版日期2010
