Determination of calcium in wine using reconfigurable PSoC based spectrophotometer

作者：Jamaludeen A^*; Neelamegam P; Rajendran A

来源：Journal of Analytical Chemistry, 2014, 69(3): 270-275.

DOI：10.1134/S106193481403006X

• calcium
• wine
• methylthymol blue
• system-on-chip
• optoelectronic instrument

This paper discusses the determination of calcium in wine samples using Programmable Systemon-Chip (PSoC) microcontroller. This embedded system uses a PSoC CY8C27443 integrated circuit (IC) spectrophotometer as core unit, high intensity orange LED as light source and photodiode as light detector having the relative spectral sensitivity above 93% in the range of 550-650 nm. This single-chip design reduces hardware complexity and increases the stability by miniaturizing the device. The instrument obeys Beer's law up to the concentration of 160 mg/L. Coloring agent Methylthymol Blue (MTB) forms a complex with calcium in presence of alkali (pH a parts per thousand 12); the complex exhibits maximum absorbance at 610 nm. The spectral range, concentration of MTB and pH of the solution at which the reaction is carried out are optimized. The results of the developed method are compared statistically with those obtained by a conventional method by applying Student's t-test and F-test. The regression analysis reveals excellent correlation (R (2) = 0.9998) and regression line equation obtained for that line Y = 0.29 + 0.99X with standard error of 0.62. It can be concluded that the obtained results are in good agreement with the reference method at 95% confidence level.

• 出版日期2014-3