A simple microwave extraction method for the isolation and identification of plant opal phytoliths

作者：Braune Caroline^*; Lieberei Reinhard; Steinmacher Douglas; Kaiser Thomas M

来源：Biologia, 2012, 67(5): 927-930.

DOI：10.2478/s11756-012-0074-1

• Arundo donax
• Phragmites australis
• extraction method
• microwave
• molecular structure of opal phytoliths
• opal phytoliths

We present a new extraction method for opal phytoliths, which has high potential to support qualitative analyses of the unaltered properties of single opal phytoliths. Opal phytoliths have to remain in their original solution or an equivalent to protect their amorphous (SiO2 nH(2)O) molecular structure. Fresh leaf material was heated in distilled water in a microwave oven for 3-4 hours, stirred and then strained through a plankton sieve (50-mu m mesh). After purification, the plant tissue matrix was successfully degraded. Single opal phytoliths of Phragmites australis and Arundo donax as well as cell debris were isolated.

• 出版日期2012-10