A novel self-assembled nano micelle as a highly efficient artificial peroxidase based on hexadecyl trimethyl ammonium bromide and cytochrome c

作者：Yang, Tian; Zhang, Yu-Shuai; Yang, Xiao-Lu; Geng, Fang-Yong; Xiao, Bao-Lin; Xu, Meng-Meng; Li, Di; Hong, Jun^*; Moosavi-Movahedi, Ali Akbar

来源：Bio-medical Materials and Engineering, 2015, 26(s1): S73-S79.

DOI：10.3233/BME-151291

• Catalytic efficiency
• artificial peroxidase
• nano-micelle
• cytochrome c
• CTAB

A novel artificial peroxidase (AP) with highly catalytic efficiency was designed using hexadecyl trimethyl ammonium bromide (CTAB, 3 mM) nano-micelles and bovine heart cytochrome c (Cyt c, 0.5 mu M) in 100 mM, pH 8.0 phosphate buffer at 25 degrees C. The catalytic rate (k(cat)) and Michaelis-Menten (K-m) of the AP were determined to be 0.311 +/- 0.013 s(-1) and 8.64 +/- 0.6 mu M. The catalytic efficiency was 0.0360 0.0020 mu M(-1)s(-1) (about 50% the efficiency of native horseradish peroxidase). The Ultraviolet visible spectrophotometer and Circular Dichroism techniques were applied to study the properties of the CTAB-Cyt c nano-micelle. Designed AP can be applied instead of native horseradish peroxidase.

• 出版日期2015
• 单位河南大学