Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine

作者：Guo, Liwei; Wang, Deyun; Hu, Yuanliang^*; Zhao, Xiaona; Wang, Yuanlei; Yang, Shujuan; Wang, Junmin; Fan, Yunpeng; Han, Guocai; Gao, Huan

来源：International Journal of Biological Macromolecules, 2012, 50(3): 512-517.

DOI：10.1016/j.ijbiomac.2012.01.002

• Astragalus polysaccharides
• Sulfated Epimedium polysaccharides
• Newcastle disease vaccine
• Avian influenza vaccine
• Immunity
• Chicken

This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides CAPS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50 mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100 mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines.

• 出版日期2012-4-1
• 单位山东农业大学; 中国农业大学; 南京农业大学