An improved PMP derivatization method for analyzing monosaccharide composition

作者：Lin Xue; Wang Zhong Fu^*; Huang Lin Juan; Bai Quan; Jia Jing Fen

来源：Chemical Journal of Chinese Universities, 2006, 27(8): 1456-1458.

• HPLC
• 1-phenyl-3-methyl-5-pyrazolone
• derivatization
• saccharide

An improved carbohydrate derivatization method employing 1-phenyl-3-methyl-5-pyrazolone(PMP) was developed in this study. Liquid ammonia was used instead of sodium hydroxide aqueous solution to provide the base medium for the derivatization reaction. Derivatization reaction and HPLC separation conditions were optimized, including reaction time, reaction temperature and PMP consumption. The derivatives of five reducing monosaccharides were well separated by HPLC. Because we employed ammonia instead of sodium hydroxide, the excessive volatile ammonia can be removed by vacumm drying, these derivatives could be directly analyzed by MALDI-TOF-MS without desalting procedure, possessing potential application for monosaccharide and oligosaccharide separation and subsequent MS analysis. This method not only increased the MS detection sensitivity, but also decreased sample wastes during purification. We successfully applied this method to monosaccharide composition analysis of polysaccharides SPPA-1, SPPB-1 and SPPC-1 purified from Spirulina Platensis.

• 出版日期2006-8-10
• 单位西北大学