Development of an in situ-Product Crystallization (ISPC)-Concept to Shift the Reaction Equilibria of Selected Amine Transaminase-Catalyzed Reactions

作者：Huelsewede Dennis; Taenzler Marco; Suess Philipp; Mildner Andrea; Menyes Ulf; von Langermann Jan^*

来源：European Journal of Organic Chemistry, 2018, 2018(18): 2130-2133.

DOI：10.1002/ejoc.201800323

• Biocatalysis
• Amine synthesis
• Product removal
• Crystallization
• Chirality

The synthesis of enantiopure amines via amine transaminases involves several challenges including unfavorable reaction equilibria and product inhibition. Described here is a non-catalytic approach to overcome such problems by using an in situ-product crystallization (ISPC) to selectively remove a targeted product amine from an amine transaminase-catalyzed reaction. The continuous removal of the product amine from its reaction solution as a barely soluble salt effectively yields a displacement of the reaction equilibrium towards the products and facilitates a simple downstream processing approach via filtration. The targeted product amine is eventually obtained from the salt, while the counterion compound can be easily recycled.

• 出版日期2018-5-15