Unveiling a Selective Mechanism for the Inhibition of -Synuclein Aggregation by -Synuclein

作者：Leitao Andre; Bhumkar Akshay; Hunter Dominic J B; Gambin Yann^*; Sierecki Emma^*

来源：International Journal of Molecular Sciences, 2018, 19(2): 334.

DOI：10.3390/ijms19020334

• alpha-synuclein
• beta-synuclein
• Parkinson's disease
• protein oligomerization
• single molecule spectroscopy
• number and brightness analysis
• two-color coincidence

alpha-Synuclein (S) is an intrinsically disordered protein that is associated with Parkinson's disease (PD) through its ability to self-assemble into oligomers and fibrils. Inhibition of this oligomerization cascade is an interesting approach to developing therapeutical strategies and -synuclein (S) has been described as a natural negative regulator of this process. However, the biological background and molecular mechanisms by which this inhibition occurs is unclear. Herein, we focused on assessing the effect of S on the aggregation of five S pathological mutants linked to early-onset PD (A30P, E46K, H50Q, G51D and A53T). By coupling single molecule fluorescence spectroscopy to a cell-free protein expression system, we validated the ability of S to act as a chaperone of S, effectively inhibiting its aggregation. Interestingly, we found that S does so in a selective manner, i.e., is a more effective inhibitor for certain S pathological mutantsA30P and G51Das compared to E46K, H50Q and A53T. Moreover, two-color coincidence experiments proved that this discrepancy is due to a preferential incorporation of S into smaller oligomers of S. This was validated by showing that the chaperoning effect was lost when proteins were mixed after being expressed individually. This study highlights the potential of fluorescence spectroscopy to deconstruct S aggregation cascade and its interplay with beta S.

• 出版日期2018-2