A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies

作者：Depieri Rogerio A; Andrade Celia G T J; Panizzi Antonio R^*

来源：Micron, 2010, 41(2): 169-171.

DOI：10.1016/j.micron.2009.09.006

• Pentatomidae
• Southern green stink bug
• Nezara viridula
• Mouthparts
• Microscopic methodology
• Cross-section
• EDTA
• Microwave irradiation

A new methodology has been developed to produce sections as thin as 5 mu m of stink bug (Heteroptera: Pentatomidae) mouthparts for morphological studies. Heads of the southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) were fixed on Bouin solution, and treated with EDTA (ethylenediamine tetraacetic acid 7%) in an ice bath within a microwave oven for 0.5, 1, 2, 3, 4, and 5 h. The chitin structures and labium soft parts were well preserved after 0.5 h EDTA treatment. Longer times of exposure to EDTA and microwave irradiation caused damage to mouthpart structures.

• 出版日期2010-2