Apoptosis induced by yessotoxins in Hela human cervical cancer cells in vitro

作者：Pang Min^*; Gao Chun Lei; Wu Zhen Xing; Lv Ning; Wang Zong Ling; Tang Xue Xi; Qu Pei

来源：Molecular Medicine Reports, 2010, 3(4): 629-634.

DOI：10.3892/mmr_00000307

• yessotoxins
• apoptosis
• Hela cells

To investigate the apoptotic effects of an extract of red-tide algae, Protoceratium reticulatum, on HeLa cells, we used liquid chromatography-tandem mass spectrometry, optical microscopy, Hoechst 33342/propidine iodide staining, and DNA gel electrophoresis to analyze its constituents and toxicity, as well as rhodamine 123 staining to investigate changes in mitochondrial membrane potential. Analysis showed that the P. reticulatum extract contained the yessotoxins (YTXs) homo-YTX, 45-OH-YTX and 45-OH-homo-YTX. The results indicated that P. reticulatum extract negatively influences HeLa cells and induces their apoptosis.

• 出版日期2010-8
• 单位中华人民共和国山东出入境检验检疫局; 国家海洋局第一海洋研究所; 中国海洋大学