Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6

作者：Wang, Kaifeng; Wu, Yue; Yin, Dan; Tang, Shixing; Hu, Guifang^*; He, Yaqing^*

来源：Archives of Virology, 2017, 162(1): 287-290.

DOI：10.1007/s00705-016-3100-8

• Recombinase polymerase amplification
• Hand, foot and mouth disease
• Coxsackievirus A6

Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.

• 出版日期2017-1
• 单位南方医科大学; 深圳市疾病预防控制中心