科研之友
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摘要
Nux-vomica is a tropical plant that contains two ergot alkaloids i.e. strychnine and brucine which have pharmaceutical and toxic properties. In some places, for example in India, Nux-vomica is used in ayurvedic preparations in homeopathic medicine. As both compounds are toxic monitoring is required. In this work a method for the chromatographic determination of strychnine and brucine in various herbal/ayurvedic sample preparations, homeopathic medicines, containing Nux-vomica, seeds of Nux-vomica as well as in spiked serum and real urine samples are described. The method involves a direct injection micellar liquid chromatography technique that was optimised under ICH guidelines. Optimum conditions are a mobile phase composed by 0.10 M SDS-pentanol 4% (v/v)-NaH2PO4 buffered at pH 3 with UV detection at 258 nm, flow rate of 1 mL min(-1), and temperature 25 degrees C. Under these conditions strychnine and brucine were analyzed in 15 and 11 min, respectively. Validation studies using ICH guidelines were performed to show that the proposed method was linear (r(2) %26gt; 0.9997), with LODs (0.18 and 0.09) and LOQs (0.69 and 0.35), for strycnine and brucine, respectively. Repeatability intra- and inter-day was below of 2.50. The developed method can be useful for analyzing strychnine and brucine for quality control and other related forensic and clinical cases.
- 出版日期2013
