A sensitive DNA biosensor fabricated with gold nanoparticles/ploy (p-aminobenzoic acid)/carbon nanotubes modified electrode

作者：Zhang, Yuzhong^*; Wang, Jie; Xu, Minglu

来源：Colloids and Surfaces B: Biointerfaces , 2010, 75(1): 179-185.

DOI：10.1016/j.colsurfb.2009.08.030

• DNA biosensor
• Multi-walled carbon nanotubes
• Gold nanoparticles
• Electropolymerization
• Electrochemical deposition

In this work, we fabricated a sensitive electrochemical DNA biosensor for the detection of target DNA. Aminobenzoic acid (ABA) was firstly electropolymerized on the surface of the glassy carbon electrode (GCE) modified with multi-walled carbon nanotubes with carboxyl groups (MWCNTs) by cyclic voltammetry (CV) Gold nanoparticles (AuNPs) were subsequently introduced to the surface of PABA-MWNTs composite film by electrochemical deposition mode. Probe DNA was immobilized on the sui face of AuNPs through Au-S bond. Scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization event by measurement of the intercalated adriamycin Under the optimal conditions, the increase of reduction peak current of adriamycin was lineal with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10(-12) to 50 x 10(-9) M with a detection limit of 35 x 10(-13) M.

• 出版日期2010-1-1
• 单位河南科技学院; 安徽师范大学