New Class 2 Integron In2-4 Among IncI1-Positive Escherichia coli Isolates Carrying ESBL and PMA beta Genes from Food Animals in Portugal

作者：Jones Dias Daniela; Manageiro Vera; Martins Ana P; Ferreira Eugenia; Canica Manuela^*

来源：Foodborne Pathogens and Disease, 2016, 13(1): 36-39.

DOI：10.1089/fpd.2015.1972

The impact of extended-spectrum -lactamases (ESBLs) and plasmid-mediated AmpC -lactamases (PMAs) of animal origin constitutes a public health concern. In this study, 179 Escherichia coli from food animals and products were analyzed, among which, 15 cephalosporin-resistant isolates harboring ESBL (CTX-M-1 [n=8], CTX-M-14 [n=1], SHV-12 [n=2]) or PMA [CMY-2, n=5]) were identified in poultry and swine, from different farms of distinct regions of Portugal. The multiple sequence-type IncI1-driven spread of ESBLs and PMAs, flanked by widely disseminated mobile elements, was guaranteed by ST26/IncI1-harboring bla(SHV-12), ST12/IncI1-harboring bla(CMY-2), ST3 and ST38/IncI1-harboring bla(CTX-M-1,) and ST1/IncI1-harboring bla(CTX-M-14). An IS10-disrupted In2-4, presenting a new attI2 recombination site, was also detected in a SHV-12/CTX-M-1-harboring isolate. This study highlights the fact that animals may act as persistent sources of ESBL- and PMA-harboring plasmids genes that might be transferred to humans through direct contact or via the food chain.

• 出版日期2016-1-1