Comparing C-60(+) and (H2O)(n)(+) clusters for mouse brain tissue analysis

作者：Razo Irma Berrueta; Sheraz Sadia; Henderson Alex; Lockyer Nicholas P; Vickerman John C^*

来源：Surface and Interface Analysis, 2014, 46(S1): 136-139.

DOI：10.1002/sia.5597

• SIMS
• tissue analysis
• cluster comparison
• H2O
• C-60
• imaging

Time-of-flight SIMS is applied to the analysis of single cells and different types of biological tissue samples enabling the generation of images with high spatial resolution and chemical specificity. However, the low yield of secondary ions from this type of sample still remains a challenge. This low yield could potentially be increased by enhancing the protonation of ions with the presence of water. Here, we have explored the application of a prototype water cluster ion beam for the analysis of mouse brain tissue samples. A series of experiments acquired with 20keV (H2O)(3000)(+) and 20keV (H2O)(4500)(+) were compared with 20keV C-60(+), showing ion yield enhancement when a (H2O)(n)(+) cluster ion is employed in the analysis. The results have demonstrated the potential benefits provided by the use of (H2O)(n)(+) clusters for the analysis of mouse brain tissue samples.

• 出版日期2014-11