DETERMINATION OF ESTIVATION-INDUCED CHANGES IN THE AMINO ACID CONTENT OF BIOMPHALARIA GLABRATA SNAILS BY HIGH PERFORMANCE THIN-LAYER CHROMATOGRAPHY-DENSITOMETRY

作者：Vasta James D; Fried Bernard^*; Sherma Joseph

来源：Journal of Liquid Chromatography & Related Technologies, 2010, 33(7-8): 1028-1037.

DOI：10.1080/10826071003766302

• alanine
• amino acids
• Biomphalaria glabrata
• estivation
• high performance thin-layer chromatography
• HPTLC
• planorbid snails
• pulmonate snails

High performance thin-layer chromatography-densitometry (HPTLC-Dens) was used to determine changes in the amino acid content of the digestive-gland gonad complex (DGG) of Biomphalaria glabrata snails as a function of estivation. Amino acids were extracted in ethanol-water (70:30) from the DGG of B. glabrata snails estivated for 7 days, and determined on silica gel or cellulose layers developed with either 2-butanol-pyridine-glacial acetic acid-deionized water (39:34:10:26) or 2-butanol-pyridine-25% ammonia-deionized water (39:34:10:26). Separated zones were detected by postchromatographic derivitization with ninhydrin and quantified by visible mode slit-scanning densitometry at 610nm. Alanine, arginine, glycine, leucine/isoleucine, lysine, serine, and valine were unequivocally identified in chromatograms from both estivated and unestivated snails. HPTLC-Dens showed a significant increase in alanine in estivated snails compared to unestivated controls (Student's t-test, P0.05). The finding of increased alanine levels in the DGG during estivation is in agreement with the hypothesis that anaerobic pathways are an important part of overall metabolism in B. glabrata during estivation.

• 出版日期2010