Association of CD30 transcripts with Th1 responses and proinflammatory cytokines in patients with end-stage renal disease

作者：Velasquez Sonia Y; Opelz Gerhard; Rojas Mauricio; Suesal Caner; Alvarez Cristiam M^*

来源：Human Immunology, 2016, 77(5): 403-410.

DOI：10.1016/j.humimm.2016.03.004

• Cytokines
• End-stage renal disease patients
• Gene expression
• Mixed lymphocyte culture
• sCD30

High serum sCD30 levels are associated with inflammatory disorders and poor outcome in renal transplantation. The contribution to these phenomena of transcripts and proteins related to CD30-activation and-cleavage is unknown. We assessed in peripheral blood of end-stage renal disease patients (ESRDP) transcripts of CD30-activation proteins CD30 and CD3OL, CD30-cleavage proteins ADAM10 and ADAM17, and Th1- and Th2-type immunity-related factors t-bet and GATA3. Additionally, we evaluated the same transcripts and release of sCD30 and 32 cytokines after allogeneic and polyclonal T-cell activation. In peripheral blood, ESRDP showed increased levels of t-bet and GATA3 transcripts compared to healthy controls (HC) (both P < 0.01) whereas levels of CD30, CD3OL, ADAM10 and ADAM17 transcripts were similar. Polyclonal and allogeneic stimulation induced higher levels of CD30 transcripts in ESRDP than in HC (both P < 0.001). Principal component analysis (PCA) in allogeneic cultures of ESRDP identified two correlation clusters, one consisting of sCD30, the Th-1 cytokine IFN-gamma, MIP-1 alpha, RANTES, sIL-2R alpha, MIP-1 beta, TNF-beta, MDC, GM-CSF and IL-5, and another one consisting of CD30 and t-bet transcripts, IL-13 and proinflammatory proteins IP-10, IL-8, IL-1R alpha and MCP-1. Reflecting an activated immune state, ESRDP exhibited after allostimulation upregulation of CD30 transcripts in T cells, which was associated with Th1 and proinflammatory responses.

• 出版日期2016-5