Adsorption behavior of ferritin and buffer components, buffer agents and salts, onto silane-coupled silicon substrate

作者：Fukuta Megumi; Yamashita Ichiro^*

来源：Colloids and Surfaces B: Biointerfaces , 2010, 75(1): 323-329.

DOI：10.1016/j.colsurfb.2009.09.004

• Ferritin
• Silane-coupling
• Adsorption mechanism
• Ion strength
• Metal contamination

Fixation of terrain using amino-silane modified substrates is effective, but salt and alkali ions of the buffer can contaminate substrates, inhibiting the sensing and fabrication of nano-elect-ionic devices To avoid adsorption of salts and alkali ions, buffer solutions have been replaced by pure water or alkaltmetal-ion-free buffer. However, proteins in such solutions are sometimes denatured Therefore, we developed a substrate which adsorbs ferritin but does not adsorb contaminants such as salts and alkali metal-ions contained in the buffer Adsorption of terrain was achieved by using a buffer with a high ion strength, such as PBS buffer, because the Debye length becomes shorter with increased ion strength clue to intermolecular force even when the substrate has no positive charge The combmation of high coverage met hyltrimethoxysilane (MTMS)-coupled silicon substrate

• 出版日期2010-1-1