AFM Study for Morphological Characteristics and Biomechanical Properties of Human Cataract Anterior Lens Capsules

作者：Choi Samjin; Lee Hui Jae; Cheong Youjin; Shin Jae Ho; Jin Kyung Hyun; Park Hun Kuk; Park Young Guk^*

来源：Scanning, 2012, 34(4): 247-256.

DOI：10.1002/sca.21001

• human anterior lens capsule
• lens epithelial cells
• surface roughness
• Young%26apos
• s modulus
• atomic force microscopy

The aim of this study was to quantitatively investigate the morphologies (surface roughness) and biomechanical properties (Young%26apos;s modulus) of human anterior lens capsules (ALCs) for noncataract and cataract groups using atomic force microscopy. Eight human ALCs obtained during phacoemulsification from patients with senile cataracts (72 +/- 13 years) were investigated in both the hydrated and dehydrated conditions. The cataract group showed clearly the proliferated lens epithelial cells (LECs) with a monomorphic cell structure, a diameter of 12.54 +/- 4.31 mu m, and a height of 0.23 +/- 0.04 mu m, whereas the control group showed no LECs. A substantial amount of false-positive calcification was observed caused by the deposition of remnants of dried salt solution. Cataract group showed significantly higher surface roughness (382.06 nm, p = 0.001) than control group in the anterior side of ALCs, whereas cataract group showed significantly lower surface roughness (353.79 nm, p = 0.001) than control group in their posterior side. Cataract group showed significantly higher Young%26apos;s modulus (69.52 kPa, p = 0.001) compared to the control group, regardless of the ALC side. Therefore, it is significant that this study provides a new method to examine the nanostructural characteristic and biomechanical property of human ALCs through a nanometer-scale resolution microscopy technique. SCANNING 34: 247-256, 2012.

• 出版日期2012-8