DETERMINATION OF PHENOLIC ACIDS IN ROOT EXUDATES OF ALLELOPATHIC RICE BY SOLID PHASE EXTRACTION-ION CHROMATOGRAPHY WITH CONDUCTIVITY DETECTION

作者：Zheng, Xinyu; Chen, Shan; Li, Qinglu; Lin, Ruiyu; Lin, Wenxiong^*

来源：Analytical Letters, 2014, 47(13): 2156-2164.

DOI：10.1080/00032719.2014.900778

• Allelochemicals
• Conductivity detection
• Ion chromatography
• Phenolic acids
• Rice root exudates

A simple, effective, and green ion chromatography method with conductivity detection was developed for the determination of benzoic acid, cinnamic acid, syringic acid, and p-hydroxybenzoic acid in the root exudates of allelopathic rice. The analytes were well separated within 25 min in an anion exchange column (150 mm x 4.0 mm i.d., 5 mu m particle size) with mixtures of 6.4 mmol L-1 Na2CO3 and 2.0 mmol L-1 NaHCO3 as the mobile phase at a flow-rate of 0.7 mL min(-1). Detection limits of benzoic acid, cinnamic acid, syringic acid, and p-hydroxybenzoic acid were 0.05, 0.20, 0.50, and 0.05 mu g mL(-1), respectively. Intra-and inter-day precision was <= 4.0% and 3.2%, respectively, and the intra- and inter-day accuracy, indicated by relative error, ranged from -8.0% to 9.0%. The developed method was successfully used to determine phenolic acids in the root exudates of allelopathic rice. The average recoveries of the analytes were between 90.7% and 103.0%.

• 出版日期2014
• 单位福建农林大学