A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses

作者：Wang, Zhengming; Hardcastle, Thomas J.; Pastor, Alex Canto; Yip, Wing Hin; Tang, Shuoya; Baulcombe, David C.^*

来源：Genes & Development, 2018, 32(17-18): 1155-1160.

DOI：10.1101/gad.313601.118

• RNA silencing
• tomato
• Dicer
• miRNA
• virus

Tomato Dicer-like2 (slDCL2) is a key component of resistance pathways against potato virus X (PVX) and tobacco mosaic virus (TMV). It is also required for production of endogenous small RNAs, including miR6026 and other non-canonical microRNAs (miRNAs). The slDCL2 mRNAs are targets of these slDCL2-dependent RNAs in a feedback loop that was disrupted by target mimic RNAs of miR6026. In lines expressing these RNAs, there was correspondingly enhanced resistance against PVX and TMV. These findings illustrate a novel miRNA pathway in plants and a crop protection strategy in which miRNA target mimicry elevates expression of defense-related mRNAs.

• 出版日期2018-9-1
• 单位香港城市大学