A novel recombinant Mycobacterium bovis bacillus Calmette-Guerin strain expressing human granulocyte macrophage colony-stimulating factor and Mycobacterium tuberculosis early secretory antigenic target 6 complex augments Th1 immunity

作者：Yang, Xiaoling; Bao, Lang^*; Deng, Yihao

来源：Acta Biochimica et Biophysica Sinica, 2011, 43(7): 511-518.

DOI：10.1093/abbs/gmr045

• GM-CSF
• ESAT6
• Th1 immunity

Since Mycobacterium bovis bacillus Calmette-Guerin strain (BCG) fails to protect adults from pulmonary tuberculosis (TB), there is an urgent need for developing a new vaccine. In this study, we constructed a novel recombinant BCG strain (rBCG) expressing human granulocyte macrophage colony-stimulating factor (GM-CSF) and the 6 kDa early secretory antigenic target (ESAT6) of Mycobacterium tuberculosis, named rBCG: GE (expressing GMCSF-ESAT6 complex), and evaluated the immunogenicity of the construct in BALB/c mice. Our results indicated that the rBCG: GE was able to induce higher titer of antibody than the conventional BCG, the rBCG: G (expressing GM-CSF) and the rBCG: E (expressing ESAT6). Moreover, the rBCG: GE also elicited a longer-lasting and stronger Th1 cellular immune responses than the other groups, which was confirmed by the incremental proliferation of splenocytes, the increased percentages of CD4(+) and CD8(+) T cells of spleen, the elevated level of interferon-gamma in splenocyte culture after tuberculin-purified protein derivative stimulation, and the increased concentration of GM-CSF in serum. The data presented here suggested the possibility that the recombinant BCG: GE might be a good vaccine candidate to TB.

• 出版日期2011-7
• 单位四川大学