Alveolar macrophage phagocytosis is impaired in children with poorly controlled asthma

作者：Fitzpatrick Anne M^*; Holguin Fernando; Teague W Gerald; Brown Lou Ann S

来源：Journal of Allergy and Clinical Immunology, 2008, 121(6): 1372-1378.

DOI：10.1016/j.jaci.2008.03.008

• asthma
• children
• innate immunity
• alveolar macrophage
• respiratory infection

Background: Lower respiratory tract infection is a differentiating feature of children with poorly controlled asthma.
Objective: Given the role of alveolar macrophages (AMs) in innate immunity, we hypothesized that AM phagocytosis might be impaired in poorly controlled asthma.
Methods: Bronchoalveolar lavage fluid AMs were isolated from 28 asthmatic children (moderate asthma, n = 12; severe asthma, n = 16), 10 nonasthmatic children with chronic cough treated with inhaled corticosteroids, and 10 healthy adult control subjects. AMs were stimulated with LPS and exposed to fluorescein isothiocyanate-conjugated Staphylococcus aureus for 2 hours. Phagocytosis was quantified by using a phagocytic index (PI) calculated from the percentage of phagocytic cells multiplied by the relative fluorescence (RFU) units of S aureus per cell. Apoptosis was determined from the percentage of cells positive for poly (adenosine diphosphate-ribose) polymerase.
Results: Phagocytosis as measured by using the unstimulated PI was decreased in subjects with poorly controlled asthma (healthy control subjects, 9330 3992 RFU; chronic cough, 9042 +/- 5976 RFU; moderate asthma, 4361 +/- 2536 RFU; severe asthma, 3153 +/- 1886 RFU; P<.001) and remained unchanged with LPS stimulation. Children with severe asthma also had increased AM apoptosis, both the unstimulated and LPS-simulated states (P<.001), which correlated with the PI.
Conclusions: AM function is compromised in children with poorly controlled asthma and is characterized by decreased phagocytosis and increased apoptosis.

• 出版日期2008-6