Morphological and morphometric analyses of crushed sciatic nerves after application of a purified protein from natural latex and hyaluronic acid hydrogel

作者：Pereira Barreiros Vanessa Cristina; Dias Fernando Jose; Iyomasa Mamie Mizusaki; Coutinho Netto Joaquim; de Sousa Luiz Gustavo; Sassoli Fazan Valeria Paula; Antunes Ricardo de Souza; Watanabe Ii sei; Mardegan Issa Joao Paulo

来源：Growth Factors, 2014, 32(5): 164-170.

DOI：10.3109/08977194.2014.952727

• Hyaluronic acid hydrogel - HAH
• peripheral nerve injury
• morphology and morphometry
• natural latex protein-F1

Hyaluronic acid hydrogels (HAHs) have been used as a carrier of substances and factors in the repair of nervous tissue. Natural latex protein (Hevea brasiliensis, F1) has shown positive effects in treating various types of tissues, including peripheral nerves. This study evaluated the F1 associated with a HAH in a controlled crush injury (axonotmesis) of the sciatic nerve in Wistar rats. The samples were photomicrographed for morphometric and quantitative analyzes using ImageJ 1.47k software (NIH, Bethesda, MD). Morphological, quantitative (myelin area/nerve area ratio and capillary density) and morphometric (minimum nerve fiber diameter, G-Ratio) data revealed an improvement in the recovery of the sciatic nerve with the application of HAH and the combination of HAH and F1 after 4 and 8 weeks of nerve injury. The most efficacious results were observed with the combination of both substances, F1 and HAH, revealing the regenerative capacity of this new biomaterial, which was hardly tested on nerve tissue.

• 出版日期2014-10