Embryo yield in llamas synchronized with two different intravaginal progesterone-releasing devices and superovulated with eCG

作者：Aller Juan F^*; Abalos Marcos C; Acuna Francisco A; Virgili Rosana; Requena Francisco; Cancino Andrea K

来源：Spanish Journal of Agricultural Research, 2015, 13(3): e04SC01.

DOI：10.5424/sjar/2015133-7308

• South American camelids
• superovulation
• hormonal treatment
• embryo

The objectives of this study were to compare the effects of two intravaginal devices (ID) containing the same dose (0.5 g) of progesterone (P-4) on subsequent ovarian response, embryo production and circulating P-4 concentration profile in llamas (Lama glama) treated with equine chorionic gonadotropin (eCG) for ovarian superstimulation. Female llamas were randomly assigned (n = 10 llamas per group) to one of the following groups and treated (Day 0) with an ID containing 0.5 g of vegetal P-4 to synchronize the emergence of a new follicular wave: i) DIB 0.5 (R) and ii) Cronipres M15 (R). On Day 3 llamas were intramuscularly treated with 1000 IU of eCG. The IDs were removed on Day 7. Llamas were naturally mated (Day 9) and treated with GnRH analogue to induce ovulation. A second mating was allowed 24 h later. Embryos were collected between 7 and 8 days after the first mating. Blood samples were taken every day from Day 0 to Day 7 to measure circulating P-4 concentrations. The results indicated that DIB device maintained greater plasma P-4 levels as compared to Cronipres until Day 2. However, the mean (+/- SD) number of corpora lutea and recovered embryos was not affected (p < 0.05) by the type of ID (5.3 +/- 2.6 vs 4.2 +/- 2.2 and 3.5 +/- 2.7 vs 2.6 +/- 3.0 for DIB and Cronipres, respectively). In conclusion, both DIB and Cronipres devices can be successfully used to synchronize the emergence of follicular wave prior to a single dose of eCG in superovulation protocol in llamas.

• 出版日期2015-9