Microbial biosensor array with transport mutants of Escherichia coli K12 for the simultaneous determination of mono-and disaccharides

作者：Held M; Schuhmann W; Jahreis K; Schmidt HL^*

来源：Biosensors and Bioelectronics, 2002, 17(11-12): 1089-1094.

DOI：10.1016/S0956-5663(02)00103-3

• whole-cell biosensor
• bacterial biosensors
• flow-injection analysis
• sensor array
• transport mutants
• sugar analysis

An automated flow-injection system with an integrated biosensor array using bacterial cells for the selective and simultaneous determination various mono- and disaccharides is described. The selectivity of the individually addressable sensors of the array was achieved by the combination of the metabolic response, measured as the O-2 consumption, of bacterial mutants of Escherichia coli K12 lacking different transport systems for individual carbohydrates. kappa-Carrageenan was used as immobilization matrix for entrapment of the bacterial cells in front of 6 individually addressable working electrodes of a screen-printed sensor array. The local consumption of molecular oxygen caused by the metabolic activity of the immobilized cells was amperometrically determined at the underlying screen-printed gold electrodes at a working potential of - 600 mV vs. Ag/AgCl. Addition of mono- or disaccharides for which functional transport systems exist in the used transport mutant strains of E coli K12 leads to an enhanced metabolic activity of the immobilized bacterial cells and to a concomitant depletion of oxygen at the electrode. Parallel determination of fructose, glucose, and sucrose was performed demonstrating the high selectivity of the proposed analytical system.

• 出版日期2002-12