BOVINE PLACENTAL DNA-POLYMERASE-DELTA - APHIDICOLIN INHIBITION AND RELATIONSHIP BETWEEN POLYMERASE AND EXONUCLEASE ACTIVITY

作者：CHO SW^*; LEE JW; CHOI SY

来源：Molecules and Cells, 1995, 5(1): 20-24.

DNA polymerase delta was purified for the first time from bovine placenta to apparent homogeneity, DNA polymerase delta is associated with a 3'- to 5'-exonuclease activity, but it is devoid of endonuclease or 5'- to 3'-exonuclease activities, The ratios of the polymerase and exonuclease activities remained constant through the purification steps, Aphidicolin inhibited both polymerase and exonuclease activities on double-stranded DNA; However, exonuclease activity on single-stranded DNA was not inhibited. The coordinated inhibition of both polymerase and exonuclease activities of DNA polymerase delta by aphidicolin is consistent with the view that its polymerase and its exonuclease activities residue on the same protein molecule. DNA polymerase delta has a total molecular mass of 178 kDa and is composed of one each of 120 kDa and 52 kDa polypeptides. The enzyme is stimulated by proliferating cell nuclear antigen to 50-fold. Other biochemical properties of the enzyme were also investigated and compared to those obtained from other sources.

• 出版日期1995-2-28