Antioxidant activity and cytoprotective effect of κ-carrageenan oligosaccharides and their different derivatives

作者：Huamao Yuan; Jinming Song; Weiwei Zhang; Xuegang Li; Ning Li; Xuelu Gao

来源：Bioorganic & Medicinal Chemistry Letters, 2006, 16(5): 1329-1334.

DOI：10.1016/j.bmcl.2005.11.057

• Carrageeenan
• Oligosaccharides
• Chemically modified derivatives
• Antioxidant
• Hydrogen peroxide
• UVA

Antioxidant activity of κ-carrageenan oligosaccharides (OM) and their chemical modification derivatives was investigated employing various established in vitro systems, such as reducing power, iron ion chelation, and total antioxidant activity using β-carotene–linoleic acid system. The oversulfated (SD), lowly (LAD), and highly acetylated derivatives (HAD) in reducing power assay, the phosphorylated derivative (PD) in metal chelating assay, and oversulfated and phosphorylated derivatives in total antioxidant activity assay exhibited antioxidant activity higher than that of carrageenan oligosaccharides. The results indicated that the chemical modification of carrageenan oligosaccharides can enhance their antioxidant activity in vitro. The protective effects of the carrageenan oligosaccharides and their chemically modified derivatives against H2O2 and UVA (long-wave ultraviolet radiation) induced oxidative damage on rat thymic lymphocyte were investigated by measuring cell viability via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Thymic lymphocyte exposure to H2O2 and UVA, a marked reduction in cell survival was observed, which was significantly prevented by carrageenan oligosaccharides and their derivatives (preincubated for 2 h) at 66.7–2000 μg/mL. But both the carrageenan oligosaccharides and their different derivatives showed the similar protective effects on intracellular level. Taken together, these results suggest that carrageenan oligosaccharides and their derivatives show relevant antioxidant activity both in vitro and in a cell system.

• 出版日期2006-3-1