Secondary structure of the 5 '-region of PGY1/MDR1 mRNA

作者：Kostenko EV; Beabealashvilly RS; Vlassov VV; Zenkova MA^*

来源：FEBS Letters, 2000, 475(3): 181-186.

DOI：10.1016/S0014-5793(00)01650-1

• RNA structure
• PGY1/MDR1
• antisense oligonucleotide

In order to identify the optimal target sites for antisense oligonucleotides in the human multiple drug resistance mRNA, the secondary structure of the 5'-terminal part of this mRNA (nucleotides 1-678) was investigated. By using results of probing with ribonucleases T1, ONE and V1 and results of computer simulations, a model of the 5'-region of the PGY1/MDR1 mRNA wits built. The molecule is formed by three major domains comprising several hairpins separated by single-stranded fragments. The predicted singl

• 出版日期2000-6-23