Morphologic and GATA1 sequencing analysis of hematopoiesis in fetuses with trisomy 21

作者：Hoeller Sylvia^*; Bihl Michel P; Tzankov Alexandar; Chaffard Rosemarie; Hirschmann Petra; Miny Peter; Kuehne Thomas; Bruder Elisabeth

来源：Human Pathology, 2014, 45(5): 1003-1009.

DOI：10.1016/j.humpath.2013.12.014

• Trisomy 21
• Hematopoiesis
• Fetal
• GATA1
• Megakaryoblastic leukaemia

Trisomy 21 alters fetal liver hematopoiesis and, in combination with somatic globin transcription factor 1 (GATA1) mutations, leads to development of transient myeloproliferative disease in newborns. However, little is known about the morphological hematopoietic changes caused by trisomy 21 in the fetus, and to date, the exact onset of GATA1 mutations remains uncertain. Therefore, we analyzed fetal liver hematopoiesis from second trimester pregnancies in trisomy 21 and screened for GATA1 mutations. We examined 57 formalin-fixed and paraffin-embedded fetal liver specimens (49 harboring trisomy 21 and 8 controls) by immunohistochemistry for CD34, CD61, factor WEE, and glycophorin A. GATA1 exon 2 was sequenced in fetal livers and corresponding nonhematologic tissue. Cell counts of megakaryocytes (P = .022), megakaryocytic precursors (P = .021), and erythroid precursors were higher in trisomy 21 cases. CD34-positive hematopoietic blasts showed no statistically significant differences. No mutation was detected by GATA1 exon 2 sequencing in fetal livers from 12 to 25 weeks of gestation. Our results suggest that GATA1 exon 2 mutations occur late in trisomy 21 fetal hematopoiesis. However, trisomy 21 alone provides a proliferative stimulus of fetal megakaryopoiesis and erythropoiesis. CD34-positive precursor cells are not increased in trisomy 21 fetal livers.

• 出版日期2014-5