Elucidated lipid structures of various human stratum corneum investigated by electron paramagnetic resonance spectroscopy

作者：Nakagawa Kouichi^*

来源：Skin Research and Technology, 2011, 17(2): 245-250.

DOI：10.1111/j.1600-0846.2010.00491.x

• EPR
• spin probe
• stratum corneum
• simulation
• lipid structure
• skin lipid
• order parameter
• ESR

Background
Electron paramagnetic resonance (EPR) in conjunction with a slow-tumbling simulation is useful for defining stratum corneum (SC) lipid structure. The objectives of this investigation were to evaluate and elucidate the lipid structure of various human SC as a function of the depth.
Methods
The SC from mid-volar forearms of human volunteers and a cadaver was stripped consecutively from one to three times using a glass plate coated with a cyanoacrylate resin. Spin probes were used to monitor SC ordering. EPR spectra were analyzed by an EPR slow-tumbling simulation. Keratin solution from human epidermis was mixed with 5-doxylstearic acid (5-DSA) aqueous solution.
Results
EPR analyses of 5-DSA and CHL probes showed immobilized and mobile spectra, respectively. The simulation for 5-DSA spectra showed differences in ordering values of the SC as a function of depth. EPR of keratin/5-DSA showed mobile spectral pattern. In addition, EPR of CHL showed a weak and a broad signal.
Conclusions
The keratin/5-DSA results imply that the most likely location of 5-DSA probe is in SC lipid. CHL probe may not easily permeate the SC. The EPR results along with the simulation analysis provide elucidated SC lipid structure.

• 出版日期2011-5