Optimization of a Method for the Profiling and Quantification of Saponins in Different Green Asparagus Genotypes

作者：Vazquez Castilla Sara; Jaramillo Carmona Sara; Maria Fuentes Alventosa Jose; Jimenez Araujo Ana; Rodriguez Arcos Rocio; Cermeno Sacristan Pedro; Antonio Espejo Calvo Juan; Guillen Bejarano Rafael^*

来源：Journal of Agricultural and Food Chemistry, 2013, 61(26): 6250-6258.

DOI：10.1021/jf401462w

• steroidal saponins
• extraction
• purification
• triguero asparagus

The main goal of this study was the optimization of a HPLC MS method for the qualitative and quantitative analysis of asparagus saponins. The method includes extraction with aqueous ethanol, cleanup by solid phase extraction, separation by reverse phase chromatography, electrospray ionization, and detection in a single quadrupole mass analyzer. The method was used for the comparison of selected genotypes of Huetor-Tajar asparagus landrace and selected varieties of commercial diploid hybrids of green asparagus. The results showed that while protodioscin was almost the only saponin detected in the commercial hybrids, eight different saponins were detected in the Huetor-Tajar asparagus genotypes. The mass spectra indicated that HT saponins are derived from a furostan type steroidal genin having a single bond between carbons 5 and 6 of the B ring. The total concentration of saponins was found to be higher in triguero asparagus than in commercial hybrids.

• 出版日期2013-7-3