Monitoring the DNA by ruthenium complexes of heterocyclic N,S-donor ligands and evaluation of biological activities

作者：Karia Parag S; Vekariya Pankajkumar A; Patidar Anshul P; Patel Ravi R; Patel Mohan N

来源：Monatshefte fur Chemie, 2016, 147(11): 1903-1914.

DOI：10.1007/s00706-016-1708-8

• N,S-donor ligands
• Ru(II)/Ru(III) complexes
• Nucleic acid interaction
• Cytotoxicity

Neutral N,S-donor bidentate ligands have been synthesized and characterized by NMR and IR spectroscopic techniques. The ligands have been used to synthesized ruthenium(II) complexes ([Ru(L-1-L-6)PPh3)(2)Cl-2]) and ruthenium(III) complexes ([Ru(L-1-L-6)PPh3)Cl-3]). Synthesized complexes have been characterized using elemental analysis, UV-Vis spectroscopy, magnetic measurements, LC-MS, and IR spectroscopy. Broth dilution technique was used to investigate antibacterial activity against two Gram-positive and three Gram-negative microorganisms and results show that all complexes are more potent than the respective ligands. UV-Vis absorption titration and viscosity measurements have been carried out to investigate the binding mode and binding strength of complexes with herring sperm DNA. The quantitative binding strength (K (b)) of the complexes was found in the range of 0.521 x 10(5)-2.94 x 10(5) M-1 and indicate intercalative mode of binding. Gel electrophoresis study was carried out to study the cleavage of pUC19 supercoiled DNA. DNA nuclease activity data for the complexes are found higher than respective ligands and metal salt. Brine shrimp bioassay was carried out to perform cytotoxicity study. IC50 values of the complexes were found in the range of 5.69 +/- 1.06-14.62 +/- 0.87 mu g cm(-3).

• 出版日期2016-11