microRNA-145 modulates migration and invasion of bladder cancer cells by targeting N-cadherin

作者：Zhang, Xue-Feng; Zhang, Xue-Qi^*; Chang, Zhe-Xing; Wu, Cui-Cui; Guo, Hang

来源：Molecular Medicine Reports, 2018, 17(6): 8450-8456.

DOI：10.3892/mmr.2018.8910

• micro RNA-145
• bladder cancer
• migration
• invasion
• N-cadherin

MicroRNA (miRNA)-145 has been demonstrated to serve a role in several types of tumors, however, the potential molecular mechanism of action of miRNA-145 in bladder cancer metastasis remains to be elucidated. This study aimed to investigate the potential modulation of miRNA-145 in bladder carcinoma and elucidate the underlying molecular mechanism. The expression of miRNA-145 in bladder adenocarcinoma tissues and bladder cancer cells was measured by reverse transcription-quantitative polymerase chain reaction. miRNA-145 mimics and inhibitor were transfected into bladder cancer (BC) cells to determine the role of miRNA-145 on cell motility and invasion measured by wound healing and transwell assays. Luciferase assay was performed to confirm whether N-cadherin was the direct target of miRNA-145. Subsequently, expression of N-cadherin and matrix metalloproteinase-9 (MMP9) in BC cells were detected by western blot analysis. miRNA-145 was significantly downregulated cells and tissues from patients with BC, compared with healthy controls. miRNA-145 markedly inhibited the ability of BC cells to migrate and invade. Furthermore, N-cadherin was identified as a target of miRNA-145 in BC cells. MMP9, acting downstream of N-cadherin, was downregulated in BC cells by miRNA-145. In the present study, miRNA-145 suppressed the migration and invasion of BC cells by regulating N-cadherin. The results of the present study indicated that miRNA-145 may function as a tumor suppressor and may have a potential to be a diagnostic and predictive biomarker, and a therapeutic target for treatment of BC.

• 出版日期2018-6
• 单位北华大学