Direct electrochemistry behavior of cytochrome c/L-cysteine modified electrode and its electrocatalytic oxidation to nitric oxide

作者：Liu Yun Chun; Cui Shi Qiang; Zhao Jun; Yang Zhou Sheng^*

来源：Bioelectrochemistry, 2007, 70(2): 416-420.

DOI：10.1016/j.bioelechem.2006.06.001

• cytochrome c
• L-cysteine
• nitric oxide
• electrocatalysis

Cytochrome c (Cyt c) was successfully immobilized on L-cysteine modified gold electrode by multicyclic voltammetry method. The electrochemical behavior of Cyt c on the L-cysteine modified electrode was explored. In 0.10 M, pH 7.0 phosphate buffer solution (PBS), Cyt c showed a quasi-reversible electrochemical redox. behavior with E-pc = 0.180 V, E-pa = 0.208 V (versus Ag/AgCl). The Cyt c/L-cysteine modified electrode gave an excellent electrocatalytic activity towards the oxidation of nitric oxide, and the catalysis currents were proportional to the nitric oxide concentration in the range of 7.0 x 10(-7) to 1.0 x 10(-5) M, the linear regression equation is 1 (mu A) = -0.124-0.003 C-NO (mu M), with a correlation coefficient 0.996, The detection limit was 3.0 x 10(-7) M (times the ratio of signal to noise, S/N=3).

• 出版日期2007-5
• 单位安徽师范大学