BONE-INDUCING ACTIVITY OF MATURE BMP-2B PRODUCED FROM A HYBRID BMP-2A/2B PRECURSOR

作者：HAMMONDS RG^*; SCHWALL R; DUDLEY A; BERKEMEIER L; LAI C; LEE J; CUNNINGHAM N; REDDI AH; WOOD WI; MASON AJ

来源：Molecular Endocrinology, 1991, 5(1): 149-155.

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2a has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

• 出版日期1991-1
• 单位NIH