Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums

作者：Nimura Akimoto; Muneta Takeshi; Otabe Koji; Koga Hideyuki; Ju Young Jin; Mochizuki Tomoyuki; Suzuki Koji; Sekiya Ichiro^*

来源：BMC Musculoskeletal Disorders, 2010, 11: 208.

DOI：10.1186/1471-2474-11-208

Background: Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.
Methods: After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.
Results: Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.
Conclusion: These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.

• 出版日期2010-9-14