Cellulase production by solid state fermentation using bagasse with Penicillium decumbens L-06

作者：Long Chuannan; Ou Yueqin; Guo Ping; Liu Yuntao; Cui Jingjing; Long Minnan; Hu Zhong^*

来源：Annals of Microbiology, 2009, 59(3): 517-523.

DOI：10.1007/bf03175140

• Penicillium decumbens
• solid-state fermentation
• cellulase
• bagasse
• Box-Behnken factorial design
• response surface methodology

The cellulase production by Penicillium decumbens L-06 in solid state fermentation (SSF) was investigated using bagasse as the substrate in this paper. The optimum conditions for cellulase production achieved by single factor testing were: the ratio of bagasse to wheat bran 1: 1 (w/w), the ratio of water to material 3: 1 (v/w), culture temperature 30 degrees C, initial pH 5.0, ammonium sulphate as nitrogen source with the concentration of 1%, 6 day's fermentation period. Box-Behnken factorial design (BBD) and response surface methodology (RSM) were further used to optimize conditions for cellulase (Filter paper activity) production. The maximal cellulase (Filter paper activity) production (3.89 FPU g(-1)) was obtained under the optimized conditions (ratio of water to material 2.38: 1, initial pH 5.28, cultivation time 150.5 h). It was well corresponded to the calculated results (3.97 FPU g(-1)) by model prediction.

• 单位
  汕头大学; 厦门大学