Downregulation of thioredoxin reductase 1 expression in the substantia nigra pars compacta of Parkinson's disease mice

作者：Liu, Zihua; Jing, Yuhong; Yin, Jie; Mu, Jiying; Yao, Tingting; Gao, Liping^*

来源：Neural Regeneration Research, 2013, 8(35): 3275-3283.

DOI：10.3969/j.issn.1673-5374.2013.35.002

• neural regeneration
• brain injury
• neurodegeneration
• Parkinson's disease
• 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• midbrain
• substantia nigra pars compacta
• tyrosine hydroxylase
• oxidative stress
• thioredoxin reductase
• grants-supported paper
• neuroregeneration

Because neurons are susceptible to oxidative damage and thioredoxin reductase 1 is extensively distributed in the central nervous system and has antioxidant properties, we speculated that the enzyme may be involved in the pathogenesis of Parkinson's disease. A Parkinson's disease model was produced by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into C57BL/6 mice. Real-time reverse transcription-PCR, western blot analysis and colorimetric assay showed that the levels of thioredoxin reductase 1 mRNA and protein were decreased, along with a significant reduction in thioredoxin reductase activity, in the midbrain of Parkinson's disease mice compared with normal mice. Immunohistochemical staining revealed that the number of thioredoxin reductase 1-positive neurons in the substantia nigra pars compacta of Parkinson's disease mice was significantly decreased compared with normal mice. These experimental findings suggest that the expression of thioredoxin reductase 1 in the substantia nigra pars compacta of Parkinson's disease mice is significantly decreased, and that the enzyme may be associated with disease onset.

• 出版日期2013-12
• 单位兰州大学